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control mab 4h2  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank control mab 4h2
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Control Mab 4h2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti rabbit secondary antibodies  (Biotium)
93
Biotium anti rabbit secondary antibodies
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Anti Rabbit Secondary Antibodies, supplied by Biotium, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal igg 1  (Proteintech)
95
Proteintech mouse monoclonal igg 1
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Mouse Monoclonal Igg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control antibody 10402 r209  (Sino Biological)
93
Sino Biological control antibody 10402 r209
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Control Antibody 10402 R209, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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loading control glyceraldehyde 3 phosphate dehydrogenase  (Proteintech)
98
Proteintech loading control glyceraldehyde 3 phosphate dehydrogenase
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Loading Control Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal igg isotype control  (Proteintech)
96
Proteintech rabbit monoclonal igg isotype control
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Rabbit Monoclonal Igg Isotype Control, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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loading control  (Proteintech)
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Proteintech loading control
(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb <t>4H2)</t> and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).
Loading Control, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb 4H2) and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).

Journal: bioRxiv

Article Title: Supracellular Mechanics and Counter-Rotational Bilateral Flows Orchestrate Posterior Morphogenesis

doi: 10.1101/2025.11.18.689090

Figure Lengend Snippet: (A) Methods used for targeted disruption of fibronectin organization and integrin-fibronectin interactions. (B) Representative phenotypes showing morphological changes following fibronectin disruption. (C) 2D max-projected confocal image of fibronectin within a 100 by 100 µm region ventral to the blastopore. (D) Morphological features of fibronectin matrix for control (mAb 4H2) and function-blocking (mAb P8D4) treatments. Each symbol represents the per-embryo mean (Mann-Whitney U, ∗p=0.0350; ∗∗p=0.0023). Bars indicate mean ± 95% CI. (E) Final frame of brightfield timelapse sequence overlaid with yellow deformation map (see also Video S7). (F) Time-projected displacement of random dot plot overlaid with vorticity. Disruptions to fibronectin result in less distinct or absent bi-directional vortices compared to controls. (G) SWIRL predicted vortex structure and spatial distribution across treatments. (H-J) Vortex characteristics across treatments. Each symbol in (H) and (I) represents a single predicted vortex: 4H2 (19 vortices from 10 embryos), P8D4 (8 from 11), COMO (21 from 13), and FNMO (18 from 13). In (J), each symbol represents an embryo with a predicted vortex pair. Vortex formation was significantly disrupted in P8D4- and FNMO-treated embryos, with a reduction in detected vortices and alterations in vortex compactness (H) and swirling strength (I). (J) Vortex asymmetry index (Mann-Whitney U, ∗p<0.0290; ∗∗p=0.0054; ∗∗p=0.0024; ∗∗∗p=0.0002). Bars indicate mean ± 95% CI, except in (H). Scale bars, 100 µm in (B,E); 20 µm in (C).

Article Snippet: To disrupt integrin binding to fibronectin, 10ml of function-blocking mAb P8D4 (19ug/μl) and control mAb 4H2 (18ug/μl) were obtained from Developmental Studies Hybridoma Bank (DSHB) and were purified using 30kDa MWCO Cytiva Protein G HP SpinTrapTM Columns (28903134; Cytiva) and concentrated using Amicon® Ultra-4 filter devices (UFC8030; Sigma), following the manufacturer’s protocol for each.

Techniques: Disruption, Control, Blocking Assay, MANN-WHITNEY, Sequencing